Genotyping in LITE is the process of determining which genetic variants are within the different populations of mosquitoes which LITE holds.
Each colony resistance colony is regularly selected with insecticide. Profiling, using discriminating doses of insecticide is performed once per year. We genotype our colonies every 5th generation to verify species and record the prevalence of known insecticide resistance alleles for quality assurance purposes.
LITE maintains close links to the Vector Biology department and when new mutations or mechanisms are identified, we screen our colonies for these new markers for further characterisation. Below is a summary of some of the major resistance mechanisms present in the LITE colonies. Further information can be found in colony fact sheets at https://doi.org/10.1186/s13071-019-3774-3
Below is a summary of some of the important resistance mechanisms that the LITE colonies hold:
| Species | Strain | Country of Origin | Kdr mutations | Metabolic Resistance |
|
Anopheles gambiae s.s. |
Kisumu | Kenya | No | No |
|
Anopheles gambiae s.l. |
Tiassalé 13 | Côte d’Ivoire | Yes | Yes |
|
Anopheles coluzzii |
VK7 2014 | Burkina Faso | Yes | Yes |
|
Anopheles funestus |
FuMOZ-R | Mozambique | No | Yes |
| FANG | Southern Angola | No | No | |
|
Anopheles arabiensis |
Moz | Mozambique | No | No |
|
Aedes aegypti |
Cayman | Grand Cayman | Yes | No |
| New Orleans | USA | No | No | |
|
Culex quinquefasciatus |
Muheza | Tanzania | No | Yes |