Colony Quality Control

Genotyping in LITE is the process of determining which genetic variants are within the different populations of mosquitoes which LITE holds. 

Each colony resistance colony is regularly selected with insecticide. Profiling, using discriminating doses of insecticide is performed once per year. We genotype our colonies every 5th generation to verify species and record the prevalence of known insecticide resistance alleles for quality assurance purposes.

LITE maintains close links to the Vector Biology department and when new mutations or mechanisms are identified, we screen our colonies for these new markers for further characterisation. Below is a summary of some of the major resistance mechanisms present in the LITE colonies.  Further information can be found in colony fact sheets at https://doi.org/10.1186/s13071-019-3774-3

Below is a summary of some of the important resistance mechanisms that the LITE colonies hold:

Species

Strain

Country of Origin

Kdr

Ace-1

Metabolic Resistance

Anopheles gambiae s.s.

Kisumu

Kenya

No

No

No

Anopheles gambiae s.I.

Tiassallé13

Côte d’Ivoire

Yes

Yes

Yes

Anopheles coluzzii

VK7 2014

Burkina Faso

Yes

No

Yes

Aedes aegypti

Cayman

Grand Cayman

Yes

No

No

New Orleans

USA

No

No

No

Anopheles arabiensis

Moz

Mozambique

No

No

No

Anopheles funestus

FUMOZ-R

Mozambique

No

No

Yes

FANG

Southern Angola

No

No

No