Analytical quantification of active ingredients using HPLC

High-performance liquid chromatography (HPLC) is used in LITE to quantify the chemical content in samples of various vector control products for quality control (QC) purposes.

LITE offers a novel miniaturised high-throughput HPLC approach (Figure 1), developed by researchers at LSTM, which is currently undergoing CIPAC validation.

Figure 1. From Walker et al. (2022) Sci Rep. Comparison between the HPLC-based CIPAC-validated methods (top) for insecticide quantification and the novel high throughput miniaturised system (bottom).

 

Advantages of this approach include:

  • Ability of quantifying two active ingredients (AIs) simultaneously
  • Miniaturised: this method uses smaller sample size and smaller quantities of solvents
  • High throughput: simultaneous processing of several samples and thus reduced run times

 

LITE can extract and quantify insecticides contained in a variety of samples, including:

  • Insecticide-treated bed nets (coated and incorporated)
  • Formulations from spray tanks used for indoor residual spraying (IRS)
  • Filter papers taken from walls sprayed with IRS
  • Insecticide-sugar solutions from Attractive Targeted Sugar Baits (ATSB)

 

The table below lists insecticides with validated methodologies but please get in touch if you have bespoke requirements you wish to discuss:

 

Insecticide

Analytical service

Permethrin

ITN QC

Alpha-cypermethrin

ITN and IRS QC

Deltamethrin

ITN and IRS QC 

Pyriproxyfen

ITN QC 

Chlorfenapyr

ITN QC 

Piperonyl butoxide

ITN QC 

Pirimiphos-methyl

ITN QC 

DDT

ITN QC 

 

Please see list of references below which report the use of the miniaturised HPLC system: